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Structured Review

Proteintech v5 tag
V5 Tag, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/v5+tag/V5-tag+Antibody/pm42875598-256-122-125
Average 95 stars, based on 145 article reviews
v5 tag - by Bioz Stars, 2026-11
95/100 stars

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Related Articles

Staining:

Article Title: A recurrent adaptive mutation in the transmembrane 2B protein of an insect picorna-like virus in a nonnative host.
Article Snippet: .. Membranes were stained with antibodies against GFP (rat, 1:1000, ChromoTek, 3h9-150), V5 tag (mouse, 1:2000, Invitrogen, 46-0705), or GAPDH (rabbit, 1:1000, Bioss, bs-8778R), followed by secondary antibodies IRDye 800CW goat anti-rat IgG (LICORBio, 926-32219), IRDye 800CW goat anti-mouse IgG (LICORBio, 926-32210), and IRDye 800CW goat anti-rabbit IgG (LICORBio, 926-32211), respectively. ..

Immunoprecipitation:

Article Title: The SHFV nsp2 and nucleocapsid proteins recruit G3BP1 to sites of viral replication, but stress granules are not induced by the infection.
Article Snippet: .. For immunoprecipitation of viral proteins with a V5 tag, the whole-cell lysates were harvested as described above and then incubated with 20 μL of V5-Trap Magnetic agarose bead slurry (Proteintech, #v5tma) at 4°C for 2 h with rotation. ..

Article Title: The SHFV nsp2 and nucleocapsid proteins recruit G3BP1 to sites of viral replication, but stress granules are not induced by the infection
Article Snippet: .. For immunoprecipitation of viral proteins with a V5 tag, the whole-cell lysates were harvested as described above and then incubated with 20 μL of V5-Trap Magnetic agarose bead slurry (Proteintech, #v5tma) at 4°C for 2 h with rotation. ..

Incubation:

Article Title: The SHFV nsp2 and nucleocapsid proteins recruit G3BP1 to sites of viral replication, but stress granules are not induced by the infection.
Article Snippet: .. For immunoprecipitation of viral proteins with a V5 tag, the whole-cell lysates were harvested as described above and then incubated with 20 μL of V5-Trap Magnetic agarose bead slurry (Proteintech, #v5tma) at 4°C for 2 h with rotation. ..

Article Title: Lipotoxic hepatocyte-derived UBQLN1 -enriched small extracellular vesicles activate hepatic stellate cells to promote hepatic fibrosis.
Article Snippet: Metabolic dysfunction-associated steatohepatitis (MASH) serves as a primary contributor to liver fibrosis, cirrhosis, and hepatocellular carcinoma, yet specific diagnostic markers and therapeutic targets remain unavailable.. This study elucidates the molecular mechanism by which UBQLN1 (ubiquilin 1) promotes MASH-related liver fibrosis by regulating small extracellular vesicles (sEVs) secretion and the functionality of the lysosome-mitochondria axis, as well as its clinical significance.. Analysis of a multicenter cohort (n = 150) demonstrated significantly elevated UBQLN1 levels in both serum and serum-derived sEV from MASH patients, exhibiting diagnostic accuracies of 0.89 and 0.95, respectively.

Article Title: The SHFV nsp2 and nucleocapsid proteins recruit G3BP1 to sites of viral replication, but stress granules are not induced by the infection
Article Snippet: .. For immunoprecipitation of viral proteins with a V5 tag, the whole-cell lysates were harvested as described above and then incubated with 20 μL of V5-Trap Magnetic agarose bead slurry (Proteintech, #v5tma) at 4°C for 2 h with rotation. ..

Article Title: Effective knockdown - replace gene therapy in a novel mouse model of DNM1 developmental and epileptic encephalopathy.
Article Snippet: .. The membranes were incubated with primary antibodies: Dynamin1- 1:200 (Invitrogen #PA1-660); V5-Tag (D3H8Q), 1:1,000 (CST #13202); ACTB, 1:15,000 (Santa Cruz Biotechnology, Dallas, TX, USA: sc-47778) overnight at 4 C, followed by incubation with secondary horseradish peroxidase-conjugated goat anti-rabbit (1:10,000) (Proteintech, Rosemont, IL, USA; SA00001-2) for 1 h at room temperature. .. Signals were developed using Amersham ECL Western Blotting Detection Reagent (GE Healthcare, Chicago, IL, USA; RPN2106) and visualized using western blot imaging system (Azure Biosystems, Dublin, CA, USA; Azure C400).

Membrane:

Article Title: Lipotoxic hepatocyte-derived UBQLN1 -enriched small extracellular vesicles activate hepatic stellate cells to promote hepatic fibrosis.
Article Snippet: Metabolic dysfunction-associated steatohepatitis (MASH) serves as a primary contributor to liver fibrosis, cirrhosis, and hepatocellular carcinoma, yet specific diagnostic markers and therapeutic targets remain unavailable.. This study elucidates the molecular mechanism by which UBQLN1 (ubiquilin 1) promotes MASH-related liver fibrosis by regulating small extracellular vesicles (sEVs) secretion and the functionality of the lysosome-mitochondria axis, as well as its clinical significance.. Analysis of a multicenter cohort (n = 150) demonstrated significantly elevated UBQLN1 levels in both serum and serum-derived sEV from MASH patients, exhibiting diagnostic accuracies of 0.89 and 0.95, respectively.

other:

Article Title: Activation of USP30 Disrupts Endothelial Cell Function and Aggravates Acute Lung Injury Through Regulating the S‐Adenosylmethionine Cycle
Article Snippet: HEK293/TLR4/MD2 cells (from InvivoGen) were cultured in DMEM medium containing 10% fetal bovine serum (FBS).

Cell-Signaling:

Article Title: USP25 augments XIAP-mediated signaling to safeguard intestinal homeostasis.
Article Snippet: Nucleotide-binding oligomerization domain containing 2 (NOD2) recognizes conserved bacterial components in the cytoplasm to initiate innate immune responses, and its mutations are closely associated with Crohn’s disease (CD).. Here, we discover that the NOD2 signaling is regulated by the deubiquitinase USP25.. Macrophages lacking USP25 exhibit impaired NOD2 signaling and are defective in cytokine production upon stimulation with muramyl dipeptide (MDP).



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